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KMID : 0613319980040030119
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Journal of the Korean Bone and Joint Tumor Soceity
1998 Volume.4 No. 3 p.119 ~ p.126
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MR Imaging and Histologic Changes in Regenerating Process of Lyophilized Hemijoint Autograft treated with Intramedullary Muscle Flap in Rabbits
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Rhee Seung-Koo
Kang Yong-Koo
Lee Joo-Yup
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Abstract
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The aim of this study was to assess whether the functional results of a hemijoint lyophilized autograft could be improved by implanting a muscle flap into the medullary cavity of autograft, and also to evaluate the applicability of MR imaging for evaluating the repair process of articular cartilage and on osteotomy site in rabbits. A hemijoint reconstruction using partially demineralized and lyophilized osteochondral autograft was done in 4 control rabbits, and combined with an intramedullary muscle flap was also done in proximal humerus of 4 rabbits. Graft healing and the repair process of osteochondral graft were followed by serial radiographs, MR imaging and histologic changes for 7 weeks after reconstruction. As a result by 5 weeks, all of control and autografts united solidly without fracture on grafted site, but their cartilages became destroyed more seriously in control group than that in autografts with muscle flap. MR imaging of control autografts showed a persistent large dead space within the marrow cavity with low signal intensity on all sequences, but in autograft with muscle flap, there are muscle obliterating the marrow cavity and intermediate signal intensities on T1-weighted images and high signal intensities on T2-weighted images. Histologically, the cartilage surface are completely destroyed and shown the severe osteoarthritic changes on all cartilage layers in control autograft,, but cartilage erosions are mild to moderate and their arthritic changes are also mild with survival of cartilage cells on deep layers. The serial MR findings of cartilage repair following autograft correlated well with the histologic findings. But the statistical significances are not confirmed in this studies due to less number of experimental animals. These results suggest that an intramedullary muscle flap can improve the functional results of hemijoint treated with partially demineralized and lyophilized osteochondral autograft by providing both vascularity and an increased populations of mesenchymal stem cells to initiate bone formation and repair in transplanted osteochondral autograft in response to osteogenhic factors residing in the autogrfts. The MR imaging can also be used very efficiently to evaluate the repair process of osteochondral graft.
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KEYWORD
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Lyophilized hemijoint autograft, Intramedullary muscle flap, MR imaging
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